Matrices listed below were spiked with certain level of RFC1 and the recovery rates were calculated by comparing the measured value to the expected amount of RFC1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
92
EDTA plasma(n=5)
85-100
91
heparin plasma(n=5)
87-104
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RFC1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
RFC1 ELISA Kit allows for the in vitro quantitative determination of RFC1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human RFC1 (Reduced Folate Carrier 1) ELISA Kit