Human Retinol binding protein 4,RBP-4 ELISA Kit

Human Retinol binding protein 4,RBP-4 ELISA Kit

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Mfr.No.

CSB-E09423h

Sensitivity

3.9 ng/mL

Detection Range

15.6 ng/mL-1000 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

retinol binding protein 4, plasma, OTTHUMP00000020116, retinol-binding protein 4, interstitial, retinol-binding protein 4, plasma, retinol binding protein 4, plasma, OTTHUMP00000020116, retinol-binding protein 4, interstitial, retinol-binding protein 4, plasma

Recovery

The recovery of human WNT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	89-94
EDTA plasma (n=4)	99	96-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human WNT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-92
1:2	Average %	97
	Range %	94-103
1:4	Average %	101
	Range %	97-106
1:8	Average %	88
	Range %	82-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.499	2.659	2.579	2.415
10	2.145	2.001	2.073	1.909
5	1.402	1.522	1.462	1.298
2.5	0.944	0.950	0.947	0.783
1.25	0.666	0.645	0.656	0.492
0.625	0.422	0.454	0.438	0.274
0.312	0.289	0.295	0.292	0.128
0	0.158	0.169	0.164

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P02753

Applications	ELISA
Research Area	RBP4

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504160	CSB-E09423h	96T	$595	Add to Cart
BHE10504160	CSB-E09423h	5x96T	$2856	Add to Cart
BHE10504160	CSB-E09423h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.