Human Retinol Binding Protein 1

Mfr.No.

E1198Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Retinol binding protein (also known as RBP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Cellular retinol-binding protein;Cellular retinol-binding protein I;CRBP;CRBP-I;RBP 1;RBP1;Retinol-binding protein 1

Reactivity

Human

Detection Range

2-600ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

15850

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.12ng/ml

Background

Cytoplasmic retinol-binding protein (PubMed: 22665496, PubMed: 26900151, PubMed: 28057518). Accepts retinol from the transport protein STRA6, and thereby contributes to retinol uptake, storage and retinoid homeostasis (PubMed: 15632377, PubMed: 22665496).

Gene ID	5947
UniProt ID	P09455
Antigen	RBP1

Research Area

Others

Protocol

These standard curves of E1198Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
400ng/ml	2.408	2.336	2.275
	2.263
200ng/ml	1.249	1.226	1.166
	1.203
100ng/ml	0.886	0.821	0.76
	0.756
50ng/ml	0.441	0.432	0.372
	0.423
25ng/ml	0.236	0.264	0.204
	0.292
0ng/ml	0.078	0.061	0
	0.043

Precision

We measured random samples of E1198Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	41.6	2.3	5.5
Intra-Assay	2	18	260.3	8.31	3.2
Intra-Assay	3	18	137.8	6.44	4.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102844	E1198Hu	48T	$320		Add to Cart
BHE12102844	E1198Hu	96T	$458		Add to Cart

Human Retinol Binding Protein 1 - RBP1 ELISA Kit

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