Human Retinoic Acid ELISA Kit

Mfr.No.

CSB-E16712h

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Retinoic Acid

Recovery

The recovery of human CPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-94
EDTA plasma (n=4)	101	95-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	105
	Range %	94-109
1:2	Average %	107
	Range %	98-111
1:4	Average %	92
	Range %	84-95
1:8	Average %	98
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	Retinoic Acid

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Soluble Mucosal Addressin Cell Adhesion Molecule 1 and Retinoic Acid are Potential Tools for Therapeutic Drug Monitoring in Patients with Inflammatory Bowel Disease Treated with Vedolizumab: A Proof of Concept Study Stephane Paul.et al,Journal of Crohn's and Colitis,May 31

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