Human Replication Initiator 1

Mfr.No.

E2401Hu

Sensitivity

10.48ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

60 kDa origin-specific DNA-binding protein;60 kDa replication initiation region protein;ATT-binding protein;DHFR oribeta-binding protein RIP60;REPIN 1;REPIN1;Replication initiator 1;Zinc finger protein 464

Molecular Weight

63575

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2401Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1649.9	48.98	3
Intra-Assay	2	18	515.9	20.09	3.9
Intra-Assay	3	18	902.6	32.9	3.6

Background

Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending.

Protocol

These standard curves of E2401Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	1.995	1.985	1.946
	1.975
1200ng/L	1.112	0.997	0.958
	0.882
600ng/L	0.604	0.587	0.547
	0.569
300ng/L	0.155	0.166	0.127
	0.176
150ng/L	0.08	0.108	0.069
	0.136
0ng/L	0.027	0.039	0
	0.051

Gene ID	29803
UniProt ID	Q9BWE0

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103939	E2401Hu	48T	$320		Add to Cart
BHE12103939	E2401Hu	96T	$458		Add to Cart

Human Replication Initiator 1 - REPIN1 ELISA Kit

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