Human Relaxin-3/RLN3 ELISA Kit PicoKine®

Mfr.No.

EK1407

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.020	0.069	0.114	0.228	0.443	0.783	1.395	2.022

Description

Human Relaxin 3 ELISA Kit PicoKine® (96 Tests). Quantitate Human RLN3 in cell culture supeatants, serum and plasma ( heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Relaxin-3;Insulin-like peptide INSL7;Insulin-like peptide 7;Prorelaxin H3;Relaxin-3 B chain;Relaxin-3 A chain;RLN3;INSL7, RXN3, ZINS4;UNQ6188/PRO20213;

Molecular Weight

15451 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D119-C142 (A) & A27-R53 (B)

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Relaxin 3

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	65	225	942	60	227	930
Standard deviation	4.42	15.07	63.11	7.7%	18.38	71.61
CV (%)	6.8%	6.7%	6.7%	8.5%	8.1%	7.7%

Protein Function

May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 (GPCR135) and relaxin-3 receptor-2 (GPCR142).

Background

Relaxin-3 is a neuropeptide which is highly conserved in species ranging from flies, fish, rodents and humans. It is a member and ancestral gene of the relaxin family of peptides, which includes the namesake hormone relaxin (designated 'H2 relaxin' in humans) which mediates peripheral actions during pregnancy and which was found to relax the pelvic ligament in guinea pigs almost a century ago. The broad distribution of relaxin-3 fibres/RXFP3 within several key neuronal circuits suggests an ability to modulate a broad range of behaviours. This has been confirmed in a growing number of rodent studies, which demonstrate relaxin-3 is able to modulate arousal, the response to stress, feeding/metabolism and memory; and likely plays a role in the generation/regulation of hippocampal theta rhythm.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	65	66	66	70	66	1.92	2.9%
Sample 2	225	219	209	235	222	9.43	4.2%
Sample 3	942	1017	854	1030	960	70.19	7.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	117579
Gene Name	RLN3
UniProt ID	Q8WXF3
Protein Name	Relaxin-3

Applications	ELISA
Research Area	Cancer,Developmental Biology,Endocrine System,Growth Factors/Hormones,Metabolism,Neurology Process,Neuropeptides,Neuroscience,Neurotransmitter,Reproduction,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001003	EK1407	96 wells/kit	$750		Add to Cart

Human Relaxin-3/RLN3 ELISA Kit PicoKine®

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