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Home / ELISA Kit

Human Relaxin 2 (RLN2) ELISA Kit

SKU : BHE13703007

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In the human there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3.RLN2 consists of an A chain and a B chain but their cleavage sites are not definitely described yet. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. Serelaxin, recombinant human relaxin-2, is a vasoactive peptide hormone with many biological and haemodynamic effects.

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Mfr.No.	KTE60791
Description	This Human Relaxin 2 (RLN2) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN2.
Alternative Names	RLN2; H2; RLXH2; bA12D24.1.1; bA12D24.1.2; prorelaxin H2; relaxin H2; relaxin; ovarian; of pregnancy
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Relaxin 2 microplate Human Relaxin 2 standard Human Relaxin 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Relaxin 2 (RLN2) ELISA Kit has high sensitivity and excellent specificity for detection of Human RLN2. No significant cross-reactivity or interference between Human RLN2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In the human there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3.RLN2 consists of an A chain and a B chain but their cleavage sites are not definitely described yet. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. Serelaxin, recombinant human relaxin-2, is a vasoactive peptide hormone with many biological and haemodynamic effects.
Notes	This Human Relaxin 2 (RLN2) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN2 in samples. An antibody specific for RLN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRLN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RLN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RLN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	6019
Antigen	RLN2
Tag	RLN2
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Relaxin 2 (RLN2) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN2 in samples. An antibody specific for RLN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRLN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RLN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RLN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703007	KTE60791	48 T	$339		Add to Cart
BHE13703007	KTE60791	96 T	$589		Add to Cart
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BHE13703007	KTE60791	96 Tx50	$24569		Add to Cart

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