Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of REL and the recovery rates were calculated by comparing the measured value to the expected amount of REL in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
97
EDTA plasma(n=5)
88-99
93
heparin plasma(n=5)
89-101
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of REL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-100%
90-102%
91-105%
EDTA plasma(n=5)
83-95%
85-99%
89-97%
heparin plasma(n=5)
83-100%
88-100%
88-98%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q04864
Antigen
REL
Applications
ELISA
Applications Notes
REL ELISA Kit allows for the in vitro quantitative determination of REL concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human REL (Proto-oncogene c-Rel) ELISA Kit