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Home / ELISA Kit

Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein (REG1A) ELISA Kit

SKU : BHE13703030

Pancreatic stone protein is the major component of the protein matrix of calculi in patients suffering from chronic calcifying pancreatitis. Secretory pancreatic stone protein is a glycoprotein in the pancreatic secretion. This protein, called PSPS, occurs in multiple molecular forms due to posttranslational processing.
The abundance of PSPS in pancreatic juice (10 to 14% of total protein) suggests that it plays an important role in exocrine pancreatic function. In vitro experiments show that PSPS inhibits CaCO(3) crystal growth. Since in all the pancreatic secretions are supersaturated in calcium carbonate, the physiologic role of PSPS may be related to its inhibitory properties. This gene encodes a protein that is secreted by the exocrine pancreas.

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Mfr.No.	KTE60814
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein (REG1A) ELISA Kit employs a two-site sandwich ELISA to quantitate REG1A.
Alternative Names	REG1A; ICRF; MGC12447; P19; PSP; PSPS; PSPS1; PTP; REG; islet cells regeneration factor; islet of langerhans regenerating protein; lithostathine 1 alpha; pancreatic stone protein; secretory; pancreatic thread
Detection Method	Colorimetric
Kit Components	Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein microplate Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein standard Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein (REG1A) ELISA Kit has high sensitivity and excellent specificity for detection of Human REG1A. No significant cross-reactivity or interference between Human REG1A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Pancreatic stone protein is the major component of the protein matrix of calculi in patients suffering from chronic calcifying pancreatitis. Secretory pancreatic stone protein is a glycoprotein in the pancreatic secretion. This protein, called PSPS, occurs in multiple molecular forms due to posttranslational processing. The abundance of PSPS in pancreatic juice (10 to 14% of total protein) suggests that it plays an important role in exocrine pancreatic function. In vitro experiments show that PSPS inhibits CaCO(3) crystal growth. Since in all the pancreatic secretions are supersaturated in calcium carbonate, the physiologic role of PSPS may be related to its inhibitory properties. This gene encodes a protein that is secreted by the exocrine pancreas.
Notes	This Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein (REG1A) ELISA Kit employs a two-site sandwich ELISA to quantitate REG1A in samples. An antibody specific for REG1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREG1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5967

Applications	ELISA
Applications Notes	This Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein (REG1A) ELISA Kit employs a two-site sandwich ELISA to quantitate REG1A in samples. An antibody specific for REG1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREG1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703030	KTE60814	48 T	$359		Add to Cart
BHE13703030	KTE60814	96 T	$619		Add to Cart
BHE13703030	KTE60814	96 Tx5	$2809		Add to Cart
BHE13703030	KTE60814	96 Tx50	$26209		Add to Cart

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