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Home / ELISA Kit

Human Reelin (RELN) ELISA Kit

SKU : BHE13703025

Reelin is a protein that helps regulate processes of neuronal migration and positioning in the developing brain. Besides this important role in early development, reelin continues to work in the adult brain. It modulates the synaptic plasticity by enhancing the induction and maintenance of long-term potentiation.
It also stimulates dendrite and dendritic spine development and regulates the continuing migration of neuroblasts generated in adult neurogenesis sites like subventricular and subgranular zones. It is found not only in the brain, but also in the spinal cord, blood, and other body organs and tissues. Reelin has been suggested to be implicated in pathogenesis of several brain diseases.

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Mfr.No.	KTE60809
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Reelin (RELN) ELISA Kit employs a two-site sandwich ELISA to quantitate RELN.
Alternative Names	RELN; PRO1598; RL; OTTHUMP00000211830
Detection Method	Colorimetric
Kit Components	Human Reelin microplate Human Reelin standard Human Reelin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Reelin (RELN) ELISA Kit has high sensitivity and excellent specificity for detection of Human RELN. No significant cross-reactivity or interference between Human RELN and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Reelin is a protein that helps regulate processes of neuronal migration and positioning in the developing brain. Besides this important role in early development, reelin continues to work in the adult brain. It modulates the synaptic plasticity by enhancing the induction and maintenance of long-term potentiation. It also stimulates dendrite and dendritic spine development and regulates the continuing migration of neuroblasts generated in adult neurogenesis sites like subventricular and subgranular zones. It is found not only in the brain, but also in the spinal cord, blood, and other body organs and tissues. Reelin has been suggested to be implicated in pathogenesis of several brain diseases.
Notes	This Human Reelin (RELN) ELISA Kit employs a two-site sandwich ELISA to quantitate RELN in samples. An antibody specific for RELN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRELN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RELN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RELN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5649

Applications	ELISA
Applications Notes	This Human Reelin (RELN) ELISA Kit employs a two-site sandwich ELISA to quantitate RELN in samples. An antibody specific for RELN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRELN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RELN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RELN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703025	KTE60809	48 T	$339		Add to Cart
BHE13703025	KTE60809	96 T	$589		Add to Cart
BHE13703025	KTE60809	96 Tx5	$2629		Add to Cart
BHE13703025	KTE60809	96 Tx50	$24569		Add to Cart

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