Human Receptor tyrosine-protein kinase erbB-3(ERBB3) ELISA kit

Human Receptor tyrosine-protein kinase erbB-3(ERBB3) ELISA kit

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Mfr.No.

CSB-EL007765HU

Sensitivity

31.25 pg/mL

Detection Range

125 pg/mL-8000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Alternative Names

v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian), ErbB-3, HER3, LCCS2, MDA-BF-1, MGC88033, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3, erbB-3, receptor tyrosine-protein kinase erbB-3, v-erb-b2 avian erythroblastic leukemia viral o

Recovery

The recovery of human NME1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	81-98
EDTA plasma (n=4)	98	90-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human NME1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	96
	Range %	84-105
1:4	Average %	95
	Range %	90-100
1:8	Average %	92
	Range %	80-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.112	2.052	2.082	1.931
600	1.849	1.706	1.778	1.627
300	1.493	1.421	1.457	1.306
150	1.021	0.993	1.007	0.856
75	0.578	0.554	0.566	0.415
37.5	0.342	0.315	0.329	0.178
18.75	0.231	0.219	0.225	0.074
0	0.155	0.147	0.151	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P21860

Applications	ELISA
Research Area	ERBB3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504126	CSB-EL007765HU	96T	$695	Add to Cart
BHE10504126	CSB-EL007765HU	5x96T	$3336	Add to Cart
BHE10504126	CSB-EL007765HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.