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Home / ELISA Kit

Human RE1-silencing transcription factor (REST) ELISA Kit

SKU : BHE13703021

This gene encodes a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described; however, their full length nature has not been determined.

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Mfr.No.	KTE60805
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human RE1-silencing transcription factor (REST) ELISA Kit employs a two-site sandwich ELISA to quantitate REST.
Alternative Names	REST; NRSF; XBR; neuron restrictive silencer factor; repressor binding to the X2 box
Detection Method	Colorimetric
Kit Components	Human RE1-silencing transcription factor microplate Human RE1-silencing transcription factor standard Human RE1-silencing transcription factor detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human RE1-silencing transcription factor (REST) ELISA Kit has high sensitivity and excellent specificity for detection of Human REST. No significant cross-reactivity or interference between Human REST and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	This gene encodes a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described; however, their full length nature has not been determined.
Notes	This Human RE1-silencing transcription factor (REST) ELISA Kit employs a two-site sandwich ELISA to quantitate REST in samples. An antibody specific for REST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REST bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5978

Applications	ELISA
Applications Notes	This Human RE1-silencing transcription factor (REST) ELISA Kit employs a two-site sandwich ELISA to quantitate REST in samples. An antibody specific for REST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REST bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703021	KTE60805	48 T	$339		Add to Cart
BHE13703021	KTE60805	96 T	$589		Add to Cart
BHE13703021	KTE60805	96 Tx5	$2629		Add to Cart
BHE13703021	KTE60805	96 Tx50	$24569		Add to Cart

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