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Home / ELISA Kit

Human RCC1 and BTB domain-containing protein 2 (RCBTB2) ELISA Kit

SKU : BHE13703032

RCBTB2 encodes a member of the RCC1-related GEF family. The N-terminal half of the encoded amino acid sequence shows similarity to the regulator of chromosome condensation RCC1, which acts as a guanine nucleotide exchange factor (GEF) protein for the Ras-related GTPase Ran.
The CHC1L gene contains 14 exons and spans at least 30 kb. The 3.2-kb CHC1L mRNA was expressed in all tissues tested by Northern blot analysis. The authors identified 4 alternatively spliced CHC1L transcripts encoding 2 protein isoforms of 551 and 526 amino acids, respectively. Sequence analysis revealed that both isoforms contain 6 repeats that share homology with repeats found in CHC1 and RPGR, and a zinc finger motif.

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Mfr.No.	KTE60816
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human RCC1 and BTB domain-containing protein 2 (RCBTB2) ELISA Kit employs a two-site sandwich ELISA to quantitate RCBTB2.
Alternative Names	RCBTB2; CHC1L; RCC1-like G exchanging factor RLG; chromosome condensation 1-like; regulator of chromosome condensation and BTB domain containing protein 2
Detection Method	Colorimetric
Kit Components	Human RCC1 and BTB domain-containing protein 2 microplate Human RCC1 and BTB domain-containing protein 2 standard Human RCC1 and BTB domain-containing protein 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human RCC1 and BTB domain-containing protein 2 (RCBTB2) ELISA Kit has high sensitivity and excellent specificity for detection of Human RCBTB2. No significant cross-reactivity or interference between Human RCBTB2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	RCBTB2 encodes a member of the RCC1-related GEF family. The N-terminal half of the encoded amino acid sequence shows similarity to the regulator of chromosome condensation RCC1, which acts as a guanine nucleotide exchange factor (GEF) protein for the Ras-related GTPase Ran. The CHC1L gene contains 14 exons and spans at least 30 kb. The 3.2-kb CHC1L mRNA was expressed in all tissues tested by Northern blot analysis. The authors identified 4 alternatively spliced CHC1L transcripts encoding 2 protein isoforms of 551 and 526 amino acids, respectively. Sequence analysis revealed that both isoforms contain 6 repeats that share homology with repeats found in CHC1 and RPGR, and a zinc finger motif.
Notes	This Human RCC1 and BTB domain-containing protein 2 (RCBTB2) ELISA Kit employs a two-site sandwich ELISA to quantitate RCBTB2 in samples. An antibody specific for RCBTB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRCBTB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RCBTB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RCBTB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	1102

Applications	ELISA
Applications Notes	This Human RCC1 and BTB domain-containing protein 2 (RCBTB2) ELISA Kit employs a two-site sandwich ELISA to quantitate RCBTB2 in samples. An antibody specific for RCBTB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRCBTB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RCBTB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RCBTB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703032	KTE60816	48 T	$339		Add to Cart
BHE13703032	KTE60816	96 T	$589		Add to Cart
BHE13703032	KTE60816	96 Tx5	$2629		Add to Cart
BHE13703032	KTE60816	96 Tx50	$24569		Add to Cart

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