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Home / ELISA Kit

Human Ras-related protein Rap-1b (RAP1B) ELISA Kit

SKU : BHE13703195

The deduced 184-amino acid protein is 95% identical to RAP1A. RAP1B shares several properties with the RAS protein, suggesting that it may bind GTP/GDP and have a membrane location.RAP1B is mainly located at the membrane, but translocates to the cytosol upon activation. In activated platelets, RAP1B interacts with the reorganized actin-based cytoskeleton. RAP1B is activated by phosphorylation, increased intracellular Ca(2+), and by agonist-induced stimulation of Gi, which results in the rapid binding of GTP to RAP1B. RAP1B is posttranslationally modified by the geranylgeranylation of cys181. Further modifications cause proteolytic removal of the last 3 C-terminal amino acids, followed by partial methylation of the remaining terminal cysteinyl carboxyl group.

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Mfr.No.	KTE60979
Description	This Human Ras-related protein Rap-1b (RAP1B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAP1B.
Alternative Names	RAP1B; DKFZp586H0723; K-REV; RAL1B; RAS-related protein RAP1B; Ras family small GTP binding protein RAP1B; small GTP binding protein
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Ras-related protein Rap-1b microplate Human Ras-related protein Rap-1b standard Human Ras-related protein Rap-1b detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Ras-related protein Rap-1b (RAP1B) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAP1B. No significant cross-reactivity or interference between Human RAP1B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The deduced 184-amino acid protein is 95% identical to RAP1A. RAP1B shares several properties with the RAS protein, suggesting that it may bind GTP/GDP and have a membrane location.RAP1B is mainly located at the membrane, but translocates to the cytosol upon activation. In activated platelets, RAP1B interacts with the reorganized actin-based cytoskeleton. RAP1B is activated by phosphorylation, increased intracellular Ca(2+), and by agonist-induced stimulation of Gi, which results in the rapid binding of GTP to RAP1B. RAP1B is posttranslationally modified by the geranylgeranylation of cys181. Further modifications cause proteolytic removal of the last 3 C-terminal amino acids, followed by partial methylation of the remaining terminal cysteinyl carboxyl group.
Notes	This Human Ras-related protein Rap-1b (RAP1B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAP1B in samples. An antibody specific for RAP1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAP1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAP1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAP1B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	5908
Antigen	RAP1B
Tag	RAP1B
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Ras-related protein Rap-1b (RAP1B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAP1B in samples. An antibody specific for RAP1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAP1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAP1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAP1B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703195	KTE60979	48 T	$339		Add to Cart
BHE13703195	KTE60979	96 T	$589		Add to Cart
BHE13703195	KTE60979	96 Tx5	$2629		Add to Cart
BHE13703195	KTE60979	96 Tx50	$24569		Add to Cart

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