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Home / ELISA Kit

Human Ras-related protein Rab-5A (RAB5A) ELISA Kit

SKU : BHE13703206

Ras-related protein Rab-5A is a protein that in humans is encoded by the RAB5A gene.RAB5A localizes to early endosomes where it is involved in the recruitment of RAB7A and the maturation of these compartments to late endosomes. It drives the maturation of endosomes by transporting vacuolar (H+)-ATPases (V-ATPases) from trans-Golgi network to endocytic vesicles.

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Mfr.No.	KTE60990
Description	This Human Ras-related protein Rab-5A (RAB5A) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB5A.
Alternative Names	RAB5A; RAB5; RAS-associated protein RAB5A
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Ras-related protein Rab-5A microplate Human Ras-related protein Rab-5A standard Human Ras-related protein Rab-5A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Ras-related protein Rab-5A (RAB5A) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAB5A. No significant cross-reactivity or interference between Human RAB5A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Ras-related protein Rab-5A is a protein that in humans is encoded by the RAB5A gene.RAB5A localizes to early endosomes where it is involved in the recruitment of RAB7A and the maturation of these compartments to late endosomes. It drives the maturation of endosomes by transporting vacuolar (H+)-ATPases (V-ATPases) from trans-Golgi network to endocytic vesicles.
Notes	This Human Ras-related protein Rab-5A (RAB5A) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB5A in samples. An antibody specific for RAB5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAB5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB5A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB5A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	5868
Antigen	RAB5A
Tag	RAB5A
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Ras-related protein Rab-5A (RAB5A) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB5A in samples. An antibody specific for RAB5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAB5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB5A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB5A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703206	KTE60990	48 T	$339		Add to Cart
BHE13703206	KTE60990	96 T	$589		Add to Cart
BHE13703206	KTE60990	96 Tx5	$2629		Add to Cart
BHE13703206	KTE60990	96 Tx50	$24569		Add to Cart

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