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Home / ELISA Kit

Human Ras-related protein Rab-3B (RAB3B) ELISA Kit

SKU : BHE13703207

The mammalian RAB proteins show striking similarities to the S. cerevisiae YPT1 and SEC4 proteins, Ras-related GTP-binding proteins involved in the regulation of secretion.RAB3B protein shares 78% identity with RAB3A.
After endocytosis, the PIGR-dIgA complex traverses several endosomal compartments and is exocytosed at the apical plasma membrane Rab3b localized to vesicular structures containing Pigr in canine kidney cells and that GTP-bound Rab3b interacted directly with the cytoplasmic domain of Pigr. Binding of dIgA to Pigr dissociated Rab3b, but a constitutively active Rab3b mutant maintained its interaction with Pigr in dIgA-treated cells. Furthermore, GTP-locked Rab3b inhibited dIgA-stimulated transcytosis.

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Mfr.No.	KTE60991
Description	This Human Ras-related protein Rab-3B (RAB3B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB3B.
Alternative Names	RAB3B; Brain antigen RAB3B
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Ras-related protein Rab-3B microplate Human Ras-related protein Rab-3B standard Human Ras-related protein Rab-3B detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Ras-related protein Rab-3B (RAB3B) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAB3B. No significant cross-reactivity or interference between Human RAB3B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The mammalian RAB proteins show striking similarities to the S. cerevisiae YPT1 and SEC4 proteins, Ras-related GTP-binding proteins involved in the regulation of secretion.RAB3B protein shares 78% identity with RAB3A. After endocytosis, the PIGR-dIgA complex traverses several endosomal compartments and is exocytosed at the apical plasma membrane Rab3b localized to vesicular structures containing Pigr in canine kidney cells and that GTP-bound Rab3b interacted directly with the cytoplasmic domain of Pigr. Binding of dIgA to Pigr dissociated Rab3b, but a constitutively active Rab3b mutant maintained its interaction with Pigr in dIgA-treated cells. Furthermore, GTP-locked Rab3b inhibited dIgA-stimulated transcytosis.
Notes	This Human Ras-related protein Rab-3B (RAB3B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB3B in samples. An antibody specific for RAB3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAB3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB3B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	5865
Antigen	RAB3B
Tag	RAB3B
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Ras-related protein Rab-3B (RAB3B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB3B in samples. An antibody specific for RAB3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAB3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB3B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703207	KTE60991	48 T	$339		Add to Cart
BHE13703207	KTE60991	96 T	$589		Add to Cart
BHE13703207	KTE60991	96 Tx5	$2629		Add to Cart
BHE13703207	KTE60991	96 Tx50	$24569		Add to Cart

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