JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / ELISA Kit

Human Ras-related protein Rab-1A (RAB1A) ELISA Kit

SKU : BHE13703209

RAB1A encodes a member of the Ras superfamily of GTPases. Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. This small GTPase controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms. RAB1 recruited p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum (ER), where it interacted with a select set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus.RAB1-regulated assembly of functional effector-SNARE complexes defines a conserved molecular mechanism to coordinate recognition between subcellular compartments.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Abbkine Scientific Co., Ltd.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	KTE60993
Description	This Human Ras-related protein Rab-1A (RAB1A) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB1A.
Alternative Names	RAB1A; DKFZp564B163; RAB1; YPT1; GTP binding protein Rab1a; RAB1; member RAS oncogene family; Rab GTPase YPT1 homolog
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Ras-related protein Rab-1A microplate Human Ras-related protein Rab-1A standard Human Ras-related protein Rab-1A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Ras-related protein Rab-1A (RAB1A) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAB1A. No significant cross-reactivity or interference between Human RAB1A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	RAB1A encodes a member of the Ras superfamily of GTPases. Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. This small GTPase controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms. RAB1 recruited p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum (ER), where it interacted with a select set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus.RAB1-regulated assembly of functional effector-SNARE complexes defines a conserved molecular mechanism to coordinate recognition between subcellular compartments.
Notes	This Human Ras-related protein Rab-1A (RAB1A) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB1A in samples. An antibody specific for RAB1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAB1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	5861
Antigen	RAB1A
Tag	RAB1A
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Ras-related protein Rab-1A (RAB1A) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB1A in samples. An antibody specific for RAB1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAB1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13703209	KTE60993	48 T	$339		Add to Cart
BHE13703209	KTE60993	96 T	$589		Add to Cart
BHE13703209	KTE60993	96 Tx5	$2629		Add to Cart
BHE13703209	KTE60993	96 Tx50	$24569		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.