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Home / ELISA Kit

Human Ras-related C3 botulinum toxin substrate 1 (RAC1) ELISA Kit

SKU : BHE13703203

Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. This mutant did bind to POR1 and it induced membrane ruffling and transformation. A second RAC mutant, which bound PAK but not POR1, induced JNK activation but was defective in inducing membrane ruffling and transformation. The authors concluded that the effects of RAC on the JNK cascade and on actin polymerization and cell proliferation are mediated by distinct effector functions that diverge at the level of RAC itself.

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Mfr.No.	KTE60987
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Ras-related C3 botulinum toxin substrate 1 (RAC1) ELISA Kit employs a two-site sandwich ELISA to quantitate RAC1.
Alternative Names	RAC1; MGC111543; MIG5; TC-25; p21-Rac1; migration-inducing gene 5; ras-related C3 botulinum toxin substrate 1; rho family; small GTP binding protein Rac1
Detection Method	Colorimetric
Kit Components	Human Ras-related C3 botulinum toxin substrate 1 microplate Human Ras-related C3 botulinum toxin substrate 1 standard Human Ras-related C3 botulinum toxin substrate 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Ras-related C3 botulinum toxin substrate 1 (RAC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAC1. No significant cross-reactivity or interference between Human RAC1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. This mutant did bind to POR1 and it induced membrane ruffling and transformation. A second RAC mutant, which bound PAK but not POR1, induced JNK activation but was defective in inducing membrane ruffling and transformation. The authors concluded that the effects of RAC on the JNK cascade and on actin polymerization and cell proliferation are mediated by distinct effector functions that diverge at the level of RAC itself.
Notes	This Human Ras-related C3 botulinum toxin substrate 1 (RAC1) ELISA Kit employs a two-site sandwich ELISA to quantitate RAC1 in samples. An antibody specific for RAC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5879

Applications	ELISA
Applications Notes	This Human Ras-related C3 botulinum toxin substrate 1 (RAC1) ELISA Kit employs a two-site sandwich ELISA to quantitate RAC1 in samples. An antibody specific for RAC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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