Matrices listed below were spiked with certain level of RAP1GAP and the recovery rates were calculated by comparing the measured value to the expected amount of RAP1GAP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
97
EDTA plasma(n=5)
90-105
98
heparin plasma(n=5)
93-103
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RAP1GAP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-105%
96-99%
86-103%
EDTA plasma(n=5)
84-100%
84-99%
84-101%
heparin plasma(n=5)
81-97%
81-99%
83-96%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P47736
Applications
ELISA
Applications Notes
RAP1GAPELISA Kit allows for the in vitro quantitative determination of RAP1GAP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human RAP1GAP(Rap1 GTPase-activating protein 1)ELISA Kit