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Home / ELISA Kit

Human Rap guanine nucleotide exchange factor 4 (RAPGEF4) ELISA Kit

SKU : BHE13703194

Sequence analysis predicted that the 1,011-amino acid cAMP-GEFII protein contains an N-terminal cAMP-binding domain and a C-terminal GEF domain. Expression of cAMP-GEFI and cAMP-GEFII activated RAP1A after forskolin and 3-isobutyl-1-methylxanthine stimulation, independent of the protein kinase A pathway.
cAMP-GEFI and cAMP-GEFII expression did not activate or only slightly activated other RAS superfamily members after stimulation. Northern blot analysis detected expression of a 4.4-kb cAMP-GEFII transcript that was most prominent in brain and adrenal gland. Within the brain, expression was strongest in cortex, occipital pole, frontal lobe, temporal lobe, amygdala, putamen, hippocampus, and cerebellum.

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Mfr.No.	KTE60978
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Rap guanine nucleotide exchange factor 4 (RAPGEF4) ELISA Kit employs a two-site sandwich ELISA to quantitate RAPGEF4.
Alternative Names	RAPGEF4; CAMP-GEFII; CGEF2; EPAC2; Nbla00496; RAP guanine-nucleotide-exchange factor (GEF) 4; cAMP-regulated guanine nucleotide exchange factor; exchange protein directly activated by cAMP 2; putative protei
Detection Method	Colorimetric
Kit Components	Human Rap guanine nucleotide exchange factor 4 microplate Human Rap guanine nucleotide exchange factor 4 standard Human Rap guanine nucleotide exchange factor 4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Rap guanine nucleotide exchange factor 4 (RAPGEF4) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAPGEF4. No significant cross-reactivity or interference between Human RAPGEF4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Sequence analysis predicted that the 1,011-amino acid cAMP-GEFII protein contains an N-terminal cAMP-binding domain and a C-terminal GEF domain. Expression of cAMP-GEFI and cAMP-GEFII activated RAP1A after forskolin and 3-isobutyl-1-methylxanthine stimulation, independent of the protein kinase A pathway. cAMP-GEFI and cAMP-GEFII expression did not activate or only slightly activated other RAS superfamily members after stimulation. Northe blot analysis detected expression of a 4.4-kb cAMP-GEFII transcript that was most prominent in brain and adrenal gland. Within the brain, expression was strongest in cortex, occipital pole, frontal lobe, temporal lobe, amygdala, putamen, hippocampus, and cerebellum.
Notes	This Human Rap guanine nucleotide exchange factor 4 (RAPGEF4) ELISA Kit employs a two-site sandwich ELISA to quantitate RAPGEF4 in samples. An antibody specific for RAPGEF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAPGEF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAPGEF4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAPGEF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	11069

Applications	ELISA
Applications Notes	This Human Rap guanine nucleotide exchange factor 4 (RAPGEF4) ELISA Kit employs a two-site sandwich ELISA to quantitate RAPGEF4 in samples. An antibody specific for RAPGEF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAPGEF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAPGEF4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAPGEF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703194	KTE60978	48 T	$339		Add to Cart
BHE13703194	KTE60978	96 T	$589		Add to Cart
BHE13703194	KTE60978	96 Tx5	$2629		Add to Cart
BHE13703194	KTE60978	96 Tx50	$24569		Add to Cart

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