Matrices listed below were spiked with certain level of RALGPS2 and the recovery rates were calculated by comparing the measured value to the expected amount of RALGPS2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
98
EDTA plasma(n=5)
85-104
95
heparin plasma(n=5)
96-102
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RALGPS2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-101%
89-101%
89-101%
EDTA plasma(n=5)
91-99%
84-100%
88-95%
heparin plasma(n=5)
82-97%
80-97%
86-95%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
Q86X27
Applications
ELISA
Applications Notes
RALGPS2 ELISA Kit allows for the in vitro quantitative determination of RALGPS2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human RALGPS2 (Ras-specific guanine nucleotide-releasing factor RalGPS2) ELISA Kit