Human Rala Binding Protein 1 - RALBP1 ELISA Kit

Mfr.No.

E2409Hu

Sensitivity

0.03ng/ml

Detection Range

0.05-40ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

76 kDa Ral-interacting protein;Dinitrophenyl S-glutathione ATPase;DNP-SG ATPase;RalA-binding protein 1;RALBP 1;RALBP1;Ral-interacting protein 1

Molecular Weight

76063

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2409Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.1	0.27	4.4
Intra-Assay	2	18	8.6	0.41	4.7
Intra-Assay	3	18	15.1	0.47	3.1

Background

Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.

Protocol

These standard curves of E2409Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.161	2.29	2.253
	2.419
12ng/ml	1.397	1.414	1.377
	1.432
6ng/ml	0.733	0.675	0.638
	0.617
3ng/ml	0.399	0.355	0.318
	0.311
1.5ng/ml	0.13	0.145	0.108
	0.159
0ng/ml	0.041	0.037	0
	0.033

Gene ID	10928
UniProt ID	Q15311

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103947	E2409Hu	48T	$320		Add to Cart
BHE12103947	E2409Hu	96T	$458		Add to Cart

Human Rala Binding Protein 1 - RALBP1 ELISA Kit

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