Matrices listed below were spiked with certain level of RAG1 and the recovery rates were calculated by comparing the measured value to the expected amount of RAG1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-102
94
EDTA plasma(n=5)
85-103
96
heparin plasma(n=5)
89-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RAG1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
RAG-1(Recombination Activating Gene 1)/RAG1/RNF74/RING finger protein 74/V(D)J recombination-activating protein 1
Notes
For Research Use Only
UniProt ID
P15918
Antigen
RAG1
Applications
ELISA
Applications Notes
RAG1J recombination-activating protein 1) ELISA Kit allows for the in vitro quantitative determination of RAG1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human RAG1(V(D)J recombination-activating protein 1) ELISA Kit