Human Radiation-inducible Immediate-Early Gene Iex-1

Mfr.No.

E6053Hu

Alternative Names

DIF2;DIF-2;Differentiation-dependent gene 2 protein;GLY96;IER3;IEX1;IEX-1;Immediate early protein GLY96;Immediate early response 3 protein;PACAP-responsive gene 1 protein;PRG1;Protein DIF-2;Protein PRG1;Radiation-inducible immediate-early gene IEX-1

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

16903

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.18ng/L

Background

May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.

Gene ID	8870
UniProt ID	P46695
Antigen	IER3

Research Area

Cell Biology; Stem Cells

Protocol

These standard curves of E6053Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.288	2.309	2.265
	2.329
1200ng/L	1.286	1.297	1.253
	1.309
600ng/L	0.853	0.883	0.839
	0.913
300ng/L	0.498	0.467	0.423
	0.437
150ng/L	0.224	0.226	0.182
	0.229
0ng/L	0.041	0.044	0
	0.047

Precision

We measured random samples of E6053Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	589.09	31.43	5.33
Intra-Assay	2	18	1699.19	56.56	3.33
Intra-Assay	3	18	237.5	16.55	6.97

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107415	E6053Hu	48T	$320		Add to Cart
BHE12107415	E6053Hu	96T	$458		Add to Cart

Human Radiation-inducible Immediate-Early Gene Iex-1 - IER3 ELISA Kit

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