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Home / ELISA Kit

Human RAC-beta serine/threonine-protein kinase (AKT2) ELISA Kit

SKU : BHE13702858

AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phosphorylating several known proteins. Mice lacking Akt2 have a normal body mass, but display a profound diabetic phenotype, indicating that Akt2 plays a key role in signal transduction downstream of the insulin receptor. Mice lacking Akt2 show worse outcome in breast cancer initaited by the large T antigen as well as the neu oncogene.

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Mfr.No.	KTE60642
Description	This Human RAC-beta serine/threonine-protein kinase (AKT2) ELISA Kit employs a two-site sandwich ELISA to quantitate AKT2.
Alternative Names	AKT2; PKBB; PKBBETA; PRKBB; RAC-BETA; AKT2 kinase; Murine thymoma viral (v-akt) homolog-2; rac protein kinase beta
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human RAC-beta serine/threonine-protein kinase microplate Human RAC-beta serine/threonine-protein kinase standard Human RAC-beta serine/threonine-protein kinase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human RAC-beta serine/threonine-protein kinase (AKT2) ELISA Kit has high sensitivity and excellent specificity for detection of Human AKT2. No significant cross-reactivity or interference between Human AKT2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phosphorylating several known proteins. Mice lacking Akt2 have a normal body mass, but display a profound diabetic phenotype, indicating that Akt2 plays a key role in signal transduction downstream of the insulin receptor. Mice lacking Akt2 show worse outcome in breast cancer initaited by the large T antigen as well as the neu oncogene.
Notes	This Human RAC-beta serine/threonine-protein kinase (AKT2) ELISA Kit employs a two-site sandwich ELISA to quantitate AKT2 in samples. An antibody specific for AKT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAKT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AKT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AKT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	208
Antigen	Akt2
Tag	AKT2
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human RAC-beta serine/threonine-protein kinase (AKT2) ELISA Kit employs a two-site sandwich ELISA to quantitate AKT2 in samples. An antibody specific for AKT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAKT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AKT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AKT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702858	KTE60642	48 T	$339		Add to Cart
BHE13702858	KTE60642	96 T	$589		Add to Cart
BHE13702858	KTE60642	96 Tx5	$2629		Add to Cart
BHE13702858	KTE60642	96 Tx50	$24569		Add to Cart

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