Human RAC-alpha serine/threonine-protein kinase(AKT1) ELISA kit

Human RAC-alpha serine/threonine-protein kinase(AKT1) ELISA kit

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Mfr.No.

CSB-EL001553HU

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

v-akt murine thymoma viral oncogene homolog 1, AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, AKT1 kinase, RAC-alpha serine/threonine-protein kinase, protein kinase B, rac protein kinase alpha

Recovery

The recovery of human PHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-97
EDTA plasma (n=4)	96	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	100
	Range %	95-108
1:4	Average %	89
	Range %	85-93
1:8	Average %	86
	Range %	81-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.593	2.502	2.548	2.387
750	2.170	2.059	2.115	1.954
375	1.397	1.419	1.408	1.247
187.5	0.781	0.825	0.803	0.642
94	0.445	0.414	0.430	0.269
47	0.273	0.258	0.266	0.105
23.5	0.226	0.212	0.219	0.058
0	0.158	0.163	0.161

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P31749

Applications	ELISA
Research Area	AKT1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504100	CSB-EL001553HU	96T	$695	Add to Cart
BHE10504100	CSB-EL001553HU	5x96T	$3336	Add to Cart
BHE10504100	CSB-EL001553HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.