Matrices listed below were spiked with certain level of RAB8B and the recovery rates were calculated by comparing the measured value to the expected amount of RAB8B in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-100
94
EDTA plasma(n=5)
88-97
91
heparin plasma(n=5)
93-104
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RAB8B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-97%
85-90%
91-102%
EDTA plasma(n=5)
82-100%
83-101%
82-99%
heparin plasma(n=5)
85-92%
84-99%
83-99%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
Q92930
Applications
ELISA
Applications Notes
RAB8BELISA Kit allows for the in vitro quantitative determination of RAB8B concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human RAB8B(Ras-related protein Rab-8B)ELISA Kit