Ras-related protein Rab-3A ELISA Kit, RAB3A ELISA Kit
Reactivity
Human
Detection Method
Double Antibody
Detection Range
31.25-2000pg/ml
Sample Type
Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of RAB3A and the recovery rates were calculated by comparing the measured value to the expected amount of RAB3A in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-96
91
EDTA plasma(n=5)
90-105
97
heparin plasma(n=5)
87-103
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RAB3A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-96%
88-105%
85-96%
EDTA plasma(n=5)
86-97%
82-98%
83-100%
heparin plasma(n=5)
86-99%
85-98%
81-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P20336
Applications
ELISA
Applications Notes
RAB3AELISA Kit allows for the in vitro quantitative determination of RAB3A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human RAB3A (Ras-related protein Rab-3A) ELISA Kit