JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / ELISA Kit

Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) ELISA Kit

SKU : BHE13703481

Koike et al. (1988) cloned and sequenced cDNAs encoding the alpha and the beta subunits. Theoretically, there may be 2 forms of pyruvate dehydrogenase deficiency, one with mutation in the PDHA gene and one with mutation in the PDHB gene. Ho et al. (1988) isolated a 1.5-kb cDNA clone for the beta subunit of E1 from a human liver gamma-gt11 cDNA library using anti-E1 serum. Using a cDNA probe, Olson et al. (1990) demonstrated that the PHE1B gene is located on 3p13-q23. Koike et al. (1990) described the molecular cloning of the entire human PHE1B gene, its characterization by restriction enzyme analysis, and its complete nucleotide sequence. The gene is composed of 10 exons and 9 introns. All intron-exon splice junctions follow the GT/AG rule. The Alu family was found in introns 2 and 8.

Continuing the saving: $50 Off All ELISA Kits Offer Extended!

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Abbkine Scientific Co., Ltd.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	KTE61265
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) ELISA Kit employs a two-site sandwich ELISA to quantitate PDHB.
Alternative Names	PDHB; DKFZp564K0164; PHE1B; Pyruvate dehydrogenase; E1 beta polypeptide
Detection Method	Colorimetric
Kit Components	Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial microplate Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial standard Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDHB. No significant cross-reactivity or interference between Human PDHB and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Koike et al. (1988) cloned and sequenced cDNAs encoding the alpha and the beta subunits. Theoretically, there may be 2 forms of pyruvate dehydrogenase deficiency, one with mutation in the PDHA gene and one with mutation in the PDHB gene. Ho et al. (1988) isolated a 1.5-kb cDNA clone for the beta subunit of E1 from a human liver gamma-gt11 cDNA library using anti-E1 serum. Using a cDNA probe, Olson et al. (1990) demonstrated that the PHE1B gene is located on 3p13-q23. Koike et al. (1990) described the molecular cloning of the entire human PHE1B gene, its characterization by restriction enzyme analysis, and its complete nucleotide sequence. The gene is composed of 10 exons and 9 introns. All intron-exon splice junctions follow the GT/AG rule. The Alu family was found in introns 2 and 8.
Notes	This Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) ELISA Kit employs a two-site sandwich ELISA to quantitate PDHB in samples. An antibody specific for PDHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDHB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5162

Applications	ELISA
Applications Notes	This Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) ELISA Kit employs a two-site sandwich ELISA to quantitate PDHB in samples. An antibody specific for PDHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDHB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13703481	KTE61265	48 T	$339		Add to Cart
BHE13703481	KTE61265	96 T	$589		Add to Cart
BHE13703481	KTE61265	96 Tx5	$2629		Add to Cart
BHE13703481	KTE61265	96 Tx50	$24569		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.