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Human Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1) ELISA Kit

SKU : BHE13703482

The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT) (E2); and dihydrolipoyl dehydrogenase (DLD) (E3). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex .Pyruvate dehydrogenase (E1) performs the first two reactions within the pyruvate dehydrogenase complex (PDC): a decarboxylation of substrate 1 (pyruvate) and a reductive acetylation of substrate 2 (lipoic acid).

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Mfr.No.	KTE61266
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1) ELISA Kit employs a two-site sandwich ELISA to quantitate PDHA1.
Alternative Names	PDHA1; RP11-723P2.1; PDHA; PDHCE1A; PHE1A; PDHE1-A type I; pyruvate dehydrogenase E1 alpha 1; pyruvate dehydrogenase E1 alpha subunit; pyruvate dehydrogenase E1 component subunit alpha; somatic form; mitochon
Detection Method	Colorimetric
Kit Components	Human Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial microplate Human Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial standard Human Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDHA1. No significant cross-reactivity or interference between Human PDHA1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT) (E2); and dihydrolipoyl dehydrogenase (DLD) (E3). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex .Pyruvate dehydrogenase (E1) performs the first two reactions within the pyruvate dehydrogenase complex (PDC): a decarboxylation of substrate 1 (pyruvate) and a reductive acetylation of substrate 2 (lipoic acid).
Notes	This Human Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1) ELISA Kit employs a two-site sandwich ELISA to quantitate PDHA1 in samples. An antibody specific for PDHA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDHA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDHA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDHA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5160

Applications	ELISA
Applications Notes	This Human Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1) ELISA Kit employs a two-site sandwich ELISA to quantitate PDHA1 in samples. An antibody specific for PDHA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDHA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDHA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDHA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703482	KTE61266	48 T	$339		Add to Cart
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