Human Pyrroline-5-carboxylate Reductase 3

Mfr.No.

E6051Hu

Sensitivity

7.02ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

P5C reductase 3;P5CR 3;PYCRL;Pyrroline-5-carboxylate reductase 3;Pyrroline-5-carboxylate reductase-like protein

Molecular Weight

28663

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6051Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	498.75	25.67	5.15
Intra-Assay	2	18	690.1	29.79	4.32
Intra-Assay	3	18	1062.82	25.98	2.44

Background

Enzyme that catalyzes the last step in proline biosynthesis. Proline is synthesized from either glutamate or ornithine; both are converted to pyrroline-5-carboxylate (P5C), and then to proline via pyrroline-5-carboxylate reductases (PYCRs). PYCRL is exclusively linked to the conversion of ornithine to proline.

Protocol

These standard curves of E6051Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.118	1.991	1.933
	1.864
800ng/L	0.91	0.913	0.855
	0.916
400ng/L	0.48	0.477	0.42
	0.475
200ng/L	0.275	0.263	0.205
	0.25
100ng/L	0.093	0.089	0.031
	0.085
0ng/L	0.058	0.058	0
	0.058

Gene ID	65263
UniProt ID	Q53H96

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107413	E6051Hu	48T	$320		Add to Cart
BHE12107413	E6051Hu	96T	$458		Add to Cart

Human Pyrroline-5-carboxylate Reductase 3 - PYCRL ELISA Kit

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