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Home / ELISA Kit

Human Pyrin domain-containing protein 2 (PYDC2) ELISA Kit

SKU : BHE13703235

Pyrin domain (PYD) proteins have recently emerged as important signaling molecules involved in the development of innate immunity against intracellular pathogens through activation of inflammatory mediator pathways. It is modulated by a cellular PYD-only protein (cPOP1), which binds to ASC and interferes with the recruitment of ASC to activated PYD-NLRs. POP (cPOP2), which shows highest homology to the PYD of PAN1. cPOP2 binds to ASC and PAN1, thereby blocking formation of cryopyrin and PAN1-containing inflammasomes, activation of caspase-1, and subsequent processing and secretion of bioactive interleukin-1beta. Existence of a second cPOP provides additional insights into inflammasome formation and suggests that POPs might be a common regulatory mechanism to "fine-tune" the activity of specific PYD-NLR family protein-containing inflammasomes.

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Mfr.No.	KTE61019
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Pyrin domain-containing protein 2 (PYDC2) ELISA Kit employs a two-site sandwich ELISA to quantitate PYDC2.
Alternative Names	PYDC2; POP2; cPOP2; pyrin-only protein 2
Detection Method	Colorimetric
Kit Components	Human Pyrin domain-containing protein 2 microplate Human Pyrin domain-containing protein 2 standard Human Pyrin domain-containing protein 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Pyrin domain-containing protein 2 (PYDC2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PYDC2. No significant cross-reactivity or interference between Human PYDC2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Pyrin domain (PYD) proteins have recently emerged as important signaling molecules involved in the development of innate immunity against intracellular pathogens through activation of inflammatory mediator pathways. It is modulated by a cellular PYD-only protein (cPOP1), which binds to ASC and interferes with the recruitment of ASC to activated PYD-NLRs. POP (cPOP2), which shows highest homology to the PYD of PAN1. cPOP2 binds to ASC and PAN1, thereby blocking formation of cryopyrin and PAN1-containing inflammasomes, activation of caspase-1, and subsequent processing and secretion of bioactive interleukin-1beta. Existence of a second cPOP provides additional insights into inflammasome formation and suggests that POPs might be a common regulatory mechanism to "fine-tune" the activity of specific PYD-NLR family protein-containing inflammasomes.
Notes	This Human Pyrin domain-containing protein 2 (PYDC2) ELISA Kit employs a two-site sandwich ELISA to quantitate PYDC2 in samples. An antibody specific for PYDC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYDC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYDC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYDC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	152138

Applications	ELISA
Applications Notes	This Human Pyrin domain-containing protein 2 (PYDC2) ELISA Kit employs a two-site sandwich ELISA to quantitate PYDC2 in samples. An antibody specific for PYDC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYDC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYDC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYDC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703235	KTE61019	48 T	$339		Add to Cart
BHE13703235	KTE61019	96 T	$589		Add to Cart
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BHE13703235	KTE61019	96 Tx50	$24569		Add to Cart

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