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Home / ELISA Kit

Human Pyrin domain-containing protein 1 (PYDC1) ELISA Kit

SKU : BHE13703236

ASC2, a PYD-only protein that functions as a modulator of multidomain PYD-containing proteins involved in NF-kappaB and caspase-1 activation. ASC2 adopts a six-helix bundle structure with a prominent loop, comprising 13 amino acid residues, between helices two and three. This loop represents a divergent feature of PYDs from other domains with the DD fold. Detailed analysis of backbone 15N NMR relaxation data using both the Lipari-Szabo model-free and reduced spectral density function formalisms revealed no evidence of contiguous stretches of polypeptide chain with dramatically increased internal motion, except at the extreme N and C termini. Some mobility in the fast, picosecond to nanosecond timescale, was seen in helix 3 and the preceding alpha2-alpha3 loop, in stark contrast to the complete disorder seen in the corresponding region of the NALP1 PYD.

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Mfr.No.	KTE61020
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Pyrin domain-containing protein 1 (PYDC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PYDC1.
Alternative Names	PYDC1; ASC2; POP1; PYC1; PAAD-only protein; pyrin domain containing 1; pyrin-domain containing protein 1; pyrin-only protein 1
Detection Method	Colorimetric
Kit Components	Human Pyrin domain-containing protein 1 microplate Human Pyrin domain-containing protein 1 standard Human Pyrin domain-containing protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Pyrin domain-containing protein 1 (PYDC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PYDC1. No significant cross-reactivity or interference between Human PYDC1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	ASC2, a PYD-only protein that functions as a modulator of multidomain PYD-containing proteins involved in NF-kappaB and caspase-1 activation. ASC2 adopts a six-helix bundle structure with a prominent loop, comprising 13 amino acid residues, between helices two and three. This loop represents a divergent feature of PYDs from other domains with the DD fold. Detailed analysis of backbone 15N NMR relaxation data using both the Lipari-Szabo model-free and reduced spectral density function formalisms revealed no evidence of contiguous stretches of polypeptide chain with dramatically increased internal motion, except at the extreme N and C termini. Some mobility in the fast, picosecond to nanosecond timescale, was seen in helix 3 and the preceding alpha2-alpha3 loop, in stark contrast to the complete disorder seen in the corresponding region of the NALP1 PYD.
Notes	This Human Pyrin domain-containing protein 1 (PYDC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PYDC1 in samples. An antibody specific for PYDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYDC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	260434

Applications	ELISA
Applications Notes	This Human Pyrin domain-containing protein 1 (PYDC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PYDC1 in samples. An antibody specific for PYDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYDC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703236	KTE61020	48 T	$339		Add to Cart
BHE13703236	KTE61020	96 T	$589		Add to Cart
BHE13703236	KTE61020	96 Tx5	$2629		Add to Cart
BHE13703236	KTE61020	96 Tx50	$24569		Add to Cart

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