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Home / ELISA Kit

Human Putative homeobox protein NANOGP8 (NANOGP8) ELISA Kit

SKU : BHE13703594

This locus is a processed pseudogene of the transcription factor NANOG. NANOG plays a central role in regulating self-renewal in pluripotent stem cells and tumor cells. This pseudogene contains an intact open reading frame that could potentially encode a protein similar to NANOG. Although there is no evidence of transcription from this pseudogene, RT-PCR studies suggest that NANOGP8 may be expressed in some cancer cell lines. In vitro studies using a recombinant NANOGP8 protein have shown that the protein localizes to the nucleus and can promote cell proliferation, similar to NANOG.

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Mfr.No.	KTE61379
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Putative homeobox protein NANOGP8 (NANOGP8) ELISA Kit employs a two-site sandwich ELISA to quantitate NANOGP8.
Alternative Names	NANOGP8; MGC119250; NANOG; NANOGP1; PN8;
Detection Method	Colorimetric
Kit Components	Human Putative homeobox protein NANOGP8 microplate Human Putative homeobox protein NANOGP8 standard Human Putative homeobox protein NANOGP8 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Putative homeobox protein NANOGP8 (NANOGP8) ELISA Kit has high sensitivity and excellent specificity for detection of Human NANOGP8. No significant cross-reactivity or interference between Human NANOGP8 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	This locus is a processed pseudogene of the transcription factor NANOG. NANOG plays a central role in regulating self-renewal in pluripotent stem cells and tumor cells. This pseudogene contains an intact open reading frame that could potentially encode a protein similar to NANOG. Although there is no evidence of transcription from this pseudogene, RT-PCR studies suggest that NANOGP8 may be expressed in some cancer cell lines. In vitro studies using a recombinant NANOGP8 protein have shown that the protein localizes to the nucleus and can promote cell proliferation, similar to NANOG.
Notes	This Human Putative homeobox protein NANOGP8 (NANOGP8) ELISA Kit employs a two-site sandwich ELISA to quantitate NANOGP8 in samples. An antibody specific for NANOGP8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNANOGP8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NANOGP8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NANOGP8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Putative homeobox protein NANOGP8 (NANOGP8) ELISA Kit employs a two-site sandwich ELISA to quantitate NANOGP8 in samples. An antibody specific for NANOGP8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNANOGP8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NANOGP8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NANOGP8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703594	KTE61379	48 T	$339		Add to Cart
BHE13703594	KTE61379	96 T	$589		Add to Cart
BHE13703594	KTE61379	96 Tx5	$2629		Add to Cart
BHE13703594	KTE61379	96 Tx50	$24569		Add to Cart

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