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Home / ELISA Kit

Human Putative helicase MOV-10 (MOV10) ELISA Kit

SKU : BHE13703787

MOV10 (Mov10 RISC Complex RNA Helicase) is a Protein Coding gene. Among its related pathways are RET signaling and Immune System. GO annotations related to this gene include poly(A) RNA binding and helicase activity. An important paralog of this gene is MOV10L1. Probable RNA helicase. Required for miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. (Microbial infection) Required for RNA-directed transcription and replication of the human hepatitis delta virus (HDV). Interacts with small capped HDV RNAs derived from genomic hairpin structures that mark the initiation sites of RNA-dependent HDV RNA transcription.

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Mfr.No.	KTE61572
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Putative helicase MOV-10 (MOV10) ELISA Kit employs a two-site sandwich ELISA to quantitate MOV10.
Alternative Names	MOV10; RP11-426L16.2; DKFZp667O1423; FLJ32791; KIAA1631; fSAP113; gb110; Mov10; Moloney leukemia virus 10; homolog; OTTHUMP00000013679; OTTHUMP00000013680; functional spliceosome-associated protein 113
Detection Method	Colorimetric
Kit Components	Human Putative helicase MOV-10 microplate Human Putative helicase MOV-10 standard Human Putative helicase MOV-10 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Putative helicase MOV-10 (MOV10) ELISA Kit has high sensitivity and excellent specificity for detection of Human MOV10. No significant cross-reactivity or interference between Human MOV10 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MOV10 (Mov10 RISC Complex RNA Helicase) is a Protein Coding gene. Among its related pathways are RET signaling and Immune System. GO annotations related to this gene include poly(A) RNA binding and helicase activity. An important paralog of this gene is MOV10L1. Probable RNA helicase. Required for miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. (Microbial infection) Required for RNA-directed transcription and replication of the human hepatitis delta virus (HDV). Interacts with small capped HDV RNAs derived from genomic hairpin structures that mark the initiation sites of RNA-dependent HDV RNA transcription.
Notes	This Human Putative helicase MOV-10 (MOV10) ELISA Kit employs a two-site sandwich ELISA to quantitate MOV10 in samples. An antibody specific for MOV10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMOV10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MOV10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MOV10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4343

Applications	ELISA
Applications Notes	This Human Putative helicase MOV-10 (MOV10) ELISA Kit employs a two-site sandwich ELISA to quantitate MOV10 in samples. An antibody specific for MOV10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMOV10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MOV10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MOV10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703787	KTE61572	48 T	$339		Add to Cart
BHE13703787	KTE61572	96 T	$589		Add to Cart
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BHE13703787	KTE61572	96 Tx50	$24569		Add to Cart

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