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Home / ELISA Kit

Human Putative E3 ubiquitin-protein ligase makorin-4 (MKRN4) ELISA Kit

SKU : BHE13703819

MKRN4P (Makorin Ring Finger Protein 4, Pseudogene) is a Pseudogene. GO annotations related to this gene include ligase activity. May act as a E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE61604
Description	This Human Putative E3 ubiquitin-protein ligase makorin-4 (MKRN4) ELISA Kit employs a two-site sandwich ELISA to quantitate MKRN4.
Alternative Names	MKRN4; MKRN4; RNF64; ZNF-Xp; ZNF127-Xp; ZNF127L1;
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Putative E3 ubiquitin-protein ligase makorin-4 microplate Human Putative E3 ubiquitin-protein ligase makorin-4 standard Human Putative E3 ubiquitin-protein ligase makorin-4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Putative E3 ubiquitin-protein ligase makorin-4 (MKRN4) ELISA Kit has high sensitivity and excellent specificity for detection of Human MKRN4. No significant cross-reactivity or interference between Human MKRN4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MKRN4P (Makorin Ring Finger Protein 4, Pseudogene) is a Pseudogene. GO annotations related to this gene include ligase activity. May act as a E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.
Notes	This Human Putative E3 ubiquitin-protein ligase makorin-4 (MKRN4) ELISA Kit employs a two-site sandwich ELISA to quantitate MKRN4 in samples. An antibody specific for MKRN4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMKRN4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MKRN4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MKRN4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Antigen	MKRN4
Tag	MKRN4
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Putative E3 ubiquitin-protein ligase makorin-4 (MKRN4) ELISA Kit employs a two-site sandwich ELISA to quantitate MKRN4 in samples. An antibody specific for MKRN4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMKRN4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MKRN4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MKRN4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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