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Home / ELISA Kit

Human Putative ATP-binding cassette sub-family C member 13 (ABCC13) ELISA Kit

SKU : BHE13703074

Putative ATP-binding cassette transporter sub-family C member 13 is a member of the superfamily of genes encoding ATP-binding cassette (ABC) transporters. This family member is part of the MRP subfamily, which is involved in multi-drug resistance, but the human locus is now thought to be a pseudogene incapable of encoding a functional ABC protein. Alternative splicing results in multiple transcript variants; however, not all variants have been fully described.
Highest expression in fetal liver and fetal spleen. In the adult, highest levels are found in the colon ascending and transverse. Also expressed in brain, placenta, lung, liver, pancreas and ovary. In bone marrow cells, levels are several fold higher than in peripheral blood leukocytes.

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Mfr.No.	KTE60858
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Putative ATP-binding cassette sub-family C member 13 (ABCC13) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCC13.
Alternative Names	ABCC13; C21orf73; PRED6; ATP-binding cassette protein C13
Detection Method	Colorimetric
Kit Components	Human Putative ATP-binding cassette sub-family C member 13 microplate Human Putative ATP-binding cassette sub-family C member 13 standard Human Putative ATP-binding cassette sub-family C member 13 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Putative ATP-binding cassette sub-family C member 13 (ABCC13) ELISA Kit has high sensitivity and excellent specificity for detection of Human ABCC13. No significant cross-reactivity or interference between Human ABCC13 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Putative ATP-binding cassette transporter sub-family C member 13 is a member of the superfamily of genes encoding ATP-binding cassette (ABC) transporters. This family member is part of the MRP subfamily, which is involved in multi-drug resistance, but the human locus is now thought to be a pseudogene incapable of encoding a functional ABC protein. Alternative splicing results in multiple transcript variants; however, not all variants have been fully described. Highest expression in fetal liver and fetal spleen. In the adult, highest levels are found in the colon ascending and transverse. Also expressed in brain, placenta, lung, liver, pancreas and ovary. In bone marrow cells, levels are several fold higher than in peripheral blood leukocytes.
Notes	This Human Putative ATP-binding cassette sub-family C member 13 (ABCC13) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCC13 in samples. An antibody specific for ABCC13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCC13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Putative ATP-binding cassette sub-family C member 13 (ABCC13) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCC13 in samples. An antibody specific for ABCC13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCC13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703074	KTE60858	48 T	$339		Add to Cart
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