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Home / ELISA Kit

Human Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (PRHOXNB) ELISA Kit

SKU : BHE13703379

PRHOXNB belongs to the OHCU decarboxylase family. Recombinant TTL from Arabidopsis thaliana catalyzes two enzymatic reactions leading to the stereoselective formation of S-allantoin, hydrolysis of hydroxyisourate through a C-terminal Urah domain, and decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline through an N-terminal Urad domain. TTL originated in green algae through a Urad-Urah fusion, which entrapped an N-terminal PTS2 between the two domains. The presence of this gene in all Viridiplantae indicates that S-allantoin biosynthesis has general significance in plant nitrogen metabolism, while conservation of alternative splicing suggests that this mechanism has general implications in the regulation of the ureide pathway in flowering plants.

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Mfr.No.	KTE61163
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (PRHOXNB) ELISA Kit employs a two-site sandwich ELISA to quantitate PRHOXNB.
Alternative Names	PRHOXNB; URAD; Parahox Cluster Neighbor
Detection Method	Colorimetric
Kit Components	Human Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase microplate Human Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase standard Human Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (PRHOXNB) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRHOXNB. No significant cross-reactivity or interference between Human PRHOXNB and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PRHOXNB belongs to the OHCU decarboxylase family. Recombinant TTL from Arabidopsis thaliana catalyzes two enzymatic reactions leading to the stereoselective formation of S-allantoin, hydrolysis of hydroxyisourate through a C-terminal Urah domain, and decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline through an N-terminal Urad domain. TTL originated in green algae through a Urad-Urah fusion, which entrapped an N-terminal PTS2 between the two domains. The presence of this gene in all Viridiplantae indicates that S-allantoin biosynthesis has general significance in plant nitrogen metabolism, while conservation of alternative splicing suggests that this mechanism has general implications in the regulation of the ureide pathway in flowering plants.
Notes	This Human Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (PRHOXNB) ELISA Kit employs a two-site sandwich ELISA to quantitate PRHOXNB in samples. An antibody specific for PRHOXNB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRHOXNB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRHOXNB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRHOXNB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	646625

Applications	ELISA
Applications Notes	This Human Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (PRHOXNB) ELISA Kit employs a two-site sandwich ELISA to quantitate PRHOXNB in samples. An antibody specific for PRHOXNB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRHOXNB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRHOXNB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRHOXNB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703379	KTE61163	48 T	$339		Add to Cart
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BHE13703379	KTE61163	96 Tx50	$24569		Add to Cart

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