Human Pulmonary Surfatcant-associated Protein D

Mfr.No.

E1072Hu

Sensitivity

0.24ng/ml

Detection Range

0.5-100ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Pulmonary Surfatcant-associated Protein D (also known as SP-D) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Collectin-7;Lung surfactant protein D;PSP-D;Pulmonary surfactant-associated protein D;SFTPD;SP-D

Molecular Weight

37728

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1072Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	17.5	0.82	4.7
Intra-Assay	2	18	55.6	2.18	3.9
Intra-Assay	3	18	27.7	1.41	5.1

Background

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

Protocol

These standard curves of E1072Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80ng/ml	2.408	2.303	2.273
	2.198
40ng/ml	1.32	1.343	1.313
	1.365
20ng/ml	0.774	0.668	0.639
	0.562
10ng/ml	0.338	0.369	0.339
	0.4
5ng/ml	0.127	0.161	0.131
	0.195
0ng/ml	0.045	0.03	0
	0.015

Gene ID	6441
UniProt ID	P35247

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102725	E1072Hu	48T	$320		Add to Cart
BHE12102725	E1072Hu	96T	$458		Add to Cart

Human Pulmonary Surfatcant-associated Protein D - SP-D ELISA Kit

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