Human Pulmonary Surfatcant-associated Protein B

Mfr.No.

E1069Hu

Sensitivity

1.12ng/ml

Detection Range

2-600ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Pulmonary Surfatcant-Associated Protein B (also known as SP-B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

18 kDa pulmonary-surfactant protein;6 kDa protein;Pulmonary surfactant-associated protein B;Pulmonary surfactant-associated proteolipid SPL(Phe;SFTPB;SP-B

Molecular Weight

42117

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1069Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	131.4	7.98	6.1
Intra-Assay	2	18	181.9	5.69	3.1
Intra-Assay	3	18	68.6	5.18	7.5

Background

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

Protocol

These standard curves of E1069Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/ml	2.281	2.34	2.27
	2.4
160ng/ml	1.216	1.326	1.256
	1.436
80ng/ml	0.761	0.828	0.758
	0.896
40ng/ml	0.549	0.532	0.461
	0.514
20ng/ml	0.319	0.279	0.208
	0.239
0ng/ml	0.062	0.07	0
	0.078

Gene ID	6439
UniProt ID	P07988

Research Area	Signal Transduction

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12102722	E1069Hu	48T	$320		Add to Cart
BHE12102722	E1069Hu	96T	$458		Add to Cart

Human Pulmonary Surfatcant-associated Protein B - SP-B ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Pulmonary Surfatcant-associated Protein B - SP-B ELISA Kit

Rate it now..!!