Human Pulmonary surfactant-associated protein D,SP-D ELISA Kit

Mfr.No.

CSB-E11166h

Sensitivity

0.31 ng/mL

Detection Range

1.25 ng/mL-80 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

surfactant protein D, COLEC7, PSP-D, SFTP4, SP-D, collectin 7, pulmonary surfactant apoprotein, pulmonary surfactant-associated protein D, surfactant, pulmonary-associated protein D, surfactant-associated protein, pulmonary

Recovery

The recovery of mouse GIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-107
EDTA plasma (n=4)	93	82-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-92
1:2	Average %	97
	Range %	94-101
1:4	Average %	102
	Range %	97-108
1:8	Average %	94
	Range %	89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.134	2.257	2.196	2.080
5	1.675	1.739	1.707	1.591
2.5	1.144	1.185	1.165	1.049
1.25	0.672	0.696	0.684	0.568
0.625	0.404	0.423	0.414	0.298
0.312	0.288	0.293	0.291	0.175
0.156	0.202	0.209	0.206	0.090
0	0.115	0.117	0.116	?

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P35247
Tissue Specificity	Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes).

Applications	ELISA
Research Area	SFTPD

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504074	CSB-E11166h	96T	$695	Add to Cart
BHE10504074	CSB-E11166h	5x96T	$3336	Add to Cart
BHE10504074	CSB-E11166h	10x96T	$6602.50	Add to Cart

Human Pulmonary surfactant-associated protein D,SP-D ELISA Kit

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