Human Pulmonary surfactant-associated protein B,SP-B ELISA Kit

Human Pulmonary surfactant-associated protein B,SP-B ELISA Kit

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Mfr.No.

CSB-E10134h

Alternative Names

surfactant protein B, PSP-B, SFTB3, SFTP3, SMDP1, SP-B, OTTHUMP00000203253, Pulmonary surfactant-associated protein B, 18kD, surfactant, pulmonary-associated protein B

Reactivity

Human

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Serum, Plasma, Urine, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of pig IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-102
EDTA plasma (n=4)	104	98-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-103
1:2	Average %	87
	Range %	81-92
1:4	Average %	95
	Range %	90-99
1:8	Average %	102
	Range %	96-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average
10	0.204	0.206	0.205
2.5	0.463	0.454	0.459
0.62	0.915	0.936	0.926
0.16	1.429	1.488	1.459
0.04	2.056	2.134	2.095
0	2.515	2.652	2.584

Species

Homo sapiens

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	P07988
Antigen	surfactant protein B

Applications

ELISA

Research Area

SFTPB

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504072	CSB-E10134h	96T	$695	Add to Cart
BHE10504072	CSB-E10134h	5x96T	$3336	Add to Cart
BHE10504072	CSB-E10134h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.