Human Pulmonary surfactant-associated protein A,SP-A ELISA Kit

Human Pulmonary surfactant-associated protein A,SP-A ELISA Kit

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Mfr.No.

CSB-E08683h

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Pulmonary surfactant-associated protein A1, AC068139.6, MGC133365, PSAP, PSPA, SFTP1, SFTPA1, pulmonary surfactant apoprotein, surfactant, pulmonary-associated protein A1, surfactant, pulmonary-associated protein A1B

Recovery

The recovery of mouse IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-96
EDTA plasma (n=4)	103	98-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	93
	Range %	88-94
1:2000	Average %	94
	Range %	90-98
1:4000	Average %	89
	Range %	85-94
1:8000	Average %	97
	Range %	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
4000	0.353	0.382	0.368
1000	0.459	0.440	0.450
500	0.541	0.507	0.524
250	0.658	0.672	0.665
62.5	1.198	1.114	1.156
0	1.964	1.903	1.934

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q8IWL2

Applications	ELISA
Research Area	SFTPA1

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10504071	CSB-E08683h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.