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Home / ELISA Kit

Human Pulmonary surfactant-associated protein A (SFTPA1) ELISA Kit

SKU : BHE13702892

SP-A (PRL) is a peptide hormone primarily associated with lactation. In breastfeeding, the infant suckling the teat stimulates the production of SP-A, which fills the breast with milk (lactogenesis) in preparation for the next feed. Oxytocin, a similar hormone, is also released, which triggers milk let-down. SP-A (PRL) is a polypeptide hormone secreted by anterior pituitary of both male and female. Release of SP-A is controlled by a complex neuroendocrine reflex initiated by a tactile stimulus and regulated by hypothalamic releasing and inhibition. Genetic variations in SFTPA1 are associated with respiratory distress syndrome in premature infants (RDS) ; also known as RDS in prematurity. RDS in the newborn is the main cause of mortality and morbidity in premature infants.

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Mfr.No.	KTE60676
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Pulmonary surfactant-associated protein A (SFTPA1) ELISA Kit employs a two-site sandwich ELISA to quantitate SFTPA1.
Alternative Names	SFTPA1; AC068139.6; MGC133365; PSAP; PSPA; SFTP1; SFTPA1; pulmonary surfactant apoprotein; surfactant; pulmonary-associated protein A1; surfactant; pulmonary-associated protein A1B
Detection Method	Colorimetric
Kit Components	Human Pulmonary surfactant-associated protein A microplate Human Pulmonary surfactant-associated protein A standard Human Pulmonary surfactant-associated protein A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Pulmonary surfactant-associated protein A (SFTPA1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SFTPA1. No significant cross-reactivity or interference between Human SFTPA1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SP-A (PRL) is a peptide hormone primarily associated with lactation. In breastfeeding, the infant suckling the teat stimulates the production of SP-A, which fills the breast with milk (lactogenesis) in preparation for the next feed. Oxytocin, a similar hormone, is also released, which triggers milk let-down. SP-A (PRL) is a polypeptide hormone secreted by anterior pituitary of both male and female. Release of SP-A is controlled by a complex neuroendocrine reflex initiated by a tactile stimulus and regulated by hypothalamic releasing and inhibition. Genetic variations in SFTPA1 are associated with respiratory distress syndrome in premature infants (RDS) ; also known as RDS in prematurity. RDS in the newborn is the main cause of mortality and morbidity in premature infants.
Notes	This Human Pulmonary surfactant-associated protein A (SFTPA1) ELISA Kit employs a two-site sandwich ELISA to quantitate SFTPA1 in samples. An antibody specific for SFTPA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFTPA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTPA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTPA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	407213

Applications	ELISA
Applications Notes	This Human Pulmonary surfactant-associated protein A (SFTPA1) ELISA Kit employs a two-site sandwich ELISA to quantitate SFTPA1 in samples. An antibody specific for SFTPA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFTPA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTPA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTPA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702892	KTE60676	48 T	$339		Add to Cart
BHE13702892	KTE60676	96 T	$589		Add to Cart
BHE13702892	KTE60676	96 Tx5	$2629		Add to Cart
BHE13702892	KTE60676	96 Tx50	$24569		Add to Cart

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