Human pulmonary activation regulated chemokine,PARC ELISA Kit

Mfr.No.

CSB-E09941h

Alternative Names

chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated), AMAC-1, AMAC1, CKb7, DC-CK1, DCCK1, MIP-4, PARC, SCYA18, CC chemokine ligand 18, alternative macrophage activation-associated CC chemokine 1, chemokine (C-C), dendritic, dendritic cell chemokine 1, macr

Reactivity

Human

Detection Range

4.69 ng/mL-300 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human BMI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-89
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BMI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-93
1:2	Average %	95
	Range %	90-100
1:4	Average %	103
	Range %	99-107
1:8	Average %	90
	Range %	85-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	1.768	1.896	1.832	1.714
800	1.169	1.203	1.186	1.068
400	0.739	0.704	0.722	0.604
200	0.472	0.457	0.465	0.347
100	0.320	0.340	0.330	0.212
50	0.267	0.271	0.269	0.151
25	0.195	0.182	0.189	0.071
0	0.121	0.115	0.118	?

Species

Homo sapiens

Sensitivity

1.17 ng/mL

Limit Of Detection

450 nm

UniProt ID	P55774
Antigen	chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)

Applications

ELISA

Research Area

CCL18

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504070	CSB-E09941h	96T	$835	Add to Cart
BHE10504070	CSB-E09941h	5x96T	$4008	Add to Cart
BHE10504070	CSB-E09941h	10x96T	$7932.50	Add to Cart

Human pulmonary activation regulated chemokine,PARC ELISA Kit

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