Matrices listed below were spiked with certain level of PTH 1-34 and the recovery rates were calculated by comparing the measured value to the expected amount of PTH 1-34 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-104
96
EDTA plasma(n=5)
85-102
94
heparin plasma(n=5)
86-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PTH 1-34 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-103%
94-100%
85-102%
EDTA plasma(n=5)
85-90%
92-101%
86-101%
heparin plasma(n=5)
92-99%
86-96%
88-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P01270
Applications
ELISA
Applications Notes
PTH 1-34 ELISA Kit allows for the in vitro quantitative determination of PTH 1-34 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PTH 1-34(Parathyroid hormone 1-34) ELISA Kit