Matrices listed below were spiked with certain level of PSPN and the recovery rates were calculated by comparing the measured value to the expected amount of PSPN in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-104
98
EDTA plasma(n=5)
89-102
95
heparin plasma(n=5)
97-105
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PSPN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PSPN ELISA Kit allows for the in vitro quantitative determination of PSPN concentrations in serum, plasma, tissue homogenates and other biological fluids.