Human Pseudomonas Exotoxin A,PEA ELISA Kit

Mfr.No.

CSB-E11252h

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Pseudomonas Exotoxin A,PEA

Recovery

The recovery of human IL31RA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	82-91
EDTA plasma (n=4)	97	92-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL31RA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	95
	Range %	89-99
1:10	Average %	102
	Range %	96-107
1:20	Average %	96
	Range %	92-103
1:40	Average %	89
	Range %	82-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	PEA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Negative pressure wound therapy reduces the motility of Pseudomonas aeruginosa and enhances wound healing in a rabbit ear biofilm infection Guoqi W.et al,Antonie Van Leeuwenhoek,Feb 21 Negative-pressure Wound Therapy in a Pseudomonas aeruginosa Infection Model Wang Guoqi.et al,/,/ Negative-Pressure Wound Therapy in a Pseudomonas aeruginosa Infection Model Guoqi Wang.et al,Biomed Res Int,May 15

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