Matrices listed below were spiked with certain level of PSAT1 and the recovery rates were calculated by comparing the measured value to the expected amount of PSAT1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
94
EDTA plasma(n=5)
84-104
95
heparin plasma(n=5)
82-105
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PSAT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-104%
83-97%
85-105%
EDTA plasma(n=5)
95-100%
90-101%
95-100%
heparin plasma(n=5)
90-98%
91-99%
91-97%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q9Y617
Applications
ELISA
Applications Notes
PSAT1 ELISA Kit allows for the in vitro quantitative determination of PSAT1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PSAT1(Phosphoserine aminotransferase) ELISA Kit