Matrices listed below were spiked with certain level of PRX and the recovery rates were calculated by comparing the measured value to the expected amount of PRX in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-100
96
EDTA plasma(n=5)
88-104
95
heparin plasma(n=5)
92-101
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PRX and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PRX ELISA Kit allows for the in vitro quantitative determination of PRX concentrations in serum, plasma, tissue homogenates and other biological fluids.